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Suchada Srifa. Improvement of homology directed repair system in chlamydomonas reinhardtii by genetic engineering. Master's Degree(Genetics). Kasetsart University. Office of the University Library. : Kasetsart University, 2017.
Improvement of homology directed repair system in chlamydomonas reinhardtii by genetic engineering
Name: Suchada Srifa
Classification :.LCCS: QH442
LCSH:
Genetic engineering
LCSH:
Genomes
LCSH:
Homology (Biology)
Abstract:
Homology directed repair (HDR) allows an incorporation of a DNA fragment into the homology site in a genome and, thereby, has been used as a mean for genome editing. Although the HDR has been used effectively in many organisms, the efficiency is of very low in most plants and microalgae. Homologous recombination (HR) plays key roles in this process through a number of enzymes required to perform in concert. Preliminary transcriptome data of Chlamydomonas reinhardtii indicated very low or no expression of many predominant HR genes such as RAD genes family, and this could be the cause of very low HDR events. This work aimed to improve the efficiency of HDR in C. reinhardtii by manipulating the RAD genes including RAD1, RAD5, RAD51, RAD52, RAD54, RAD55, RAD57 and DMC1. Coding sequences of the RAD genes were cloned from yeast and expressed in C. reinhardtii under the control of a constitutive promoter. Transformants were analyzed by western blot for successful of protein expression. C. reinhardtii transformants expressing the eight genes were tested for HDR event by transformation using a mutated version of Phytoene synthase (PSY), which is responsible for carotenoid biosynthesis. Transformants with HDR events was expected to exhibit white colony, while the ectopic integrations remain green. However, this approach failed to improve the HDR event. In addition, UV irradiation or high light intensity treatment was found to up-regulate the expression of RAD genes in C. reinhardtii. This observation may help improving the efficiency of HDR system microalgae.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Role:
Thesis Advisor
Name: Anchalee Sirikhachornkit
Role:
Thesis Co-Advisor
Name: Anongpat Suttangkakul
Role:
Thesis Co-Advisor
Created:
2017
Modified:
2023-06-29
Issued:
2023-06-29
วิทยานิพนธ์/Thesis
application/pdf
URL: https://kasetsart.idm.oclc.org/login?url=https://www.lib.ku.ac.th/KUthesis/2560/suchada-sri-all.pdf
CallNumber:
QH442.S92
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Genetics
Grantor: Kasetsart University
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
0.038452 วินาที
Suchada Srifa
| Title | Contributor | Type |
|---|---|---|
| Improvement of homology directed repair system in chlamydomonas reinhardtii by genetic engineering
มหาวิทยาลัยเกษตรศาสตร์ Suchada Srifa | Supachai Vuttipongchaikij Anchalee Sirikhachornkit Anongpat Suttangkakul | วิทยานิพนธ์/Thesis |
Supachai Vuttipongchaikij
| Title | Creator | Type and Date Create |
|---|---|---|
| Improvement of homology directed repair system in chlamydomonas reinhardtii by genetic engineering
มหาวิทยาลัยเกษตรศาสตร์ Supachai Vuttipongchaikij ;Anchalee Sirikhachornkit;Anongpat Suttangkakul | Suchada Srifa | วิทยานิพนธ์/Thesis |
| Characterization of leafy cotyledon1 (LEC1) in oil palm (Elaeis guineensis Jacq)
มหาวิทยาลัยเกษตรศาสตร์ Chatchawan Jantasuriyarat;Supachai Vuttipongchaikij | Mantira Suksirt | วิทยานิพนธ์/Thesis |
Anchalee Sirikhachornkit
| Title | Creator | Type and Date Create |
|---|---|---|
| Improvement of homology directed repair system in chlamydomonas reinhardtii by genetic engineering
มหาวิทยาลัยเกษตรศาสตร์ Supachai Vuttipongchaikij ;Anchalee Sirikhachornkit;Anongpat Suttangkakul | Suchada Srifa | วิทยานิพนธ์/Thesis |
| Improving the content of high value lipids in the green algae chlamydomonas reinhardtii
มหาวิทยาลัยเกษตรศาสตร์ Anchalee Sirikhachornkit | Supakorn Potijun | วิทยานิพนธ์/Thesis |
| Development of unicellular microalgae for carotenoid production
มหาวิทยาลัยเกษตรศาสตร์ Anchalee Sirikhachornkit | Thanaporn Intha | วิทยานิพนธ์/Thesis |
| Natural variation of high light tolerant phenotype in the green alga chlamydomonas reinhardtii
มหาวิทยาลัยเกษตรศาสตร์ Anchalee Sirikhachornkit | Nittaya Suwannachuen | วิทยานิพนธ์/Thesis |
| Characterization of high light tolerant mutants of Chlamydomonas reinhardtii under oxidative stress
มหาวิทยาลัยเกษตรศาสตร์ Anchalee Sirikhachornkit | Chonlada Yaisamlee | วิทยานิพนธ์/Thesis |
Anongpat Suttangkakul